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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

Cytokeratin 20 was originally identified as IT, a low molecular weight, acidic (Type I) cytokeratin. This antibody can be used in immunohistochemistry as a marker for a restricted profile of carcinomas, including adenocarcinomas of the colon, transitional cell carcinomas of the bladder, Merkel-cell tumors of the skin, mucinous varian carcinoma, and some pancreatic carcinomas. Squamous cell carcinoma, adenocarcinomas of the lung, and breast, endometrium and neuroendocrine tumors were essentially negative.
Catalog #	C9097-29E
Applications	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections.
	Optimal dilutions to be determined by the researcher.
Positive Control	Adenocarcinoma of the colon
	Expected Staining Pattern: Cytoplasmic
	Epitope Retrieval: Required (Citrate Buffer pH 6.0)
	Enzyme Digestion: Required (Pepsin)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6A60
Host	Mouse
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.1% sodium azide. before the addition of glycerol to 40%.
Purity	Culture supernatant
Immunogen	Cytokeratin 20 isolated from human duodenal mucosa.
Specificity	Recognizes low molecular weight cytokeratin 20 (46kD). Shows no cross reactivity to other cytokeratin subtypes and intermediate filament types, including vimentin, desmin, glial fibrillary acidic protein and neurofilament protein
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.