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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 20

Anti -Cytokeratin 20

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Cytokeratin 20 was originally identified as IT, a low molecular weight, acidic (Type I) cytokeratin. This antibody can be used in immunohistochemistry as a marker for a restricted profile of carcinomas, including adenocarcinomas of the colon, transitional cell carcinomas of the bladder, Merkel-cell tumors of the skin, mucinous varian carcinoma, and some pancreatic carcinomas. Squamous cell carcinoma, adenocarcinomas of the lung, and breast, endometrium and neuroendocrine tumors were essentially negative.
Catalog #C9097-29E
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.
Positive Control Adenocarcinoma of the colon
Expected Staining Pattern: Cytoplasmic
Epitope Retrieval: Required (Citrate Buffer pH 6.0)
Enzyme Digestion: Required (Pepsin)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A60
HostMouse
SourceHuman
ConcentrationNot determined.
FormSupplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.1% sodium azide. before the addition of glycerol to 40%.
PurityCulture supernatant
ImmunogenCytokeratin 20 isolated from human duodenal mucosa.
SpecificityRecognizes low molecular weight cytokeratin 20 (46kD). Shows no cross reactivity to other cytokeratin subtypes and intermediate filament types, including vimentin, desmin, glial fibrillary acidic protein and neurofilament protein
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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