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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 2e

Anti -Cytokeratin 2e


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Clone Host Grade Applications
Monoclonal Mouse Supernatant E B IH IF
Keratins are a group of water-insoluble proteins that form monofilaments, a class of intermediate filament. These filaments form part of the cytoskeletal complex in epidermis and in most other epithelial tissues. Nineteen human epithelial keratins are resolved with two-dimensional gels electrophoresis (1). These can be divided into acid (pI < 5.7) and basic (pI > 6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50, 48, 46, 45 and 40kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kD. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state (2-4). The 56.5/65-67kD pair is present in keratinized (differentiated) epidermis. The 55/64kD pair is characteristic of normal (corneal-type) epithelial differentiation (1,2). The 51/59kD pair is characteristic of the stratified squamous epithelial of internal organism such as esophagus and tongue (1,3). The 51/58kD pair is a keratinocyte marker; this pair is present in almost all stratified epithelia irrespective of the state of cellular stratification (1,2). The 48/56kD pair is characteristic of hyperproliferative (de-differentiated) keratinocytes (1,5). The 45/52kD pair and to a lesser extent, the 46/54kD pair are characteristic of simple epithelia (1). The 40kD keratin is present in most epithelia except adult epidermis (1).
Catalog #C9097-33
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry. Excellent marker to study terminal epidermal differentiation. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:100. Note: Paraffin sections require antigen retrieval with microwave.
Frozen sections require antigen retrieval by pretreatment with PBS, 0.2% Triton-X100 for 5 minutes.
Immunohistochemistry: 1:200 (PAP/ABC)
Immunofluorescence: 1:200
Western Blot: 1:200 (alkaline phosphatase)
Western Blot: 1:60,000 (ECL)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5D49
ConcentrationNot determined
FormSupplied as a liquid, tissue culture supernatant.
PurityTissue culture supernatant
ImmunogenSynthetic peptide corresponding to the N-terminal aa2-23 of human cytokeratin 2e (MW 65,852).
SpecificityReactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. Recognizes individual cells within epidermis of tongue and mamille (co-localization with cytokeratin 10). Reactive on hyperkeratosis of diverse viral and genetic origin. Does not crossreact with palate cytokeratin 2p. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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