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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 2e

Anti -Cytokeratin 2e


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
This clone represents an excellent marker to study terminal epidermal differentiation.
Catalog #C9097-33A
ApplicationsSuitable for use in Immunoblot and Immunohistochemistry (frozen sections, pretreatment with Triton-X100 is recommended, e.g. 0.2% in PBS for 5 min.; On paraffin sections the microwave method is recommended).
Recommended DilutionWestern Blot: 1:200 (alkaline phosphatase)
Western Blot: 1:60,000 (ECL)
Immunofluorescence: 1:200
Immunohistochemistry: 1:100 (PAP/ABC)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5F231
ConcentrationNot determined
FormSupplied as a liquid.
PurityHybridoma culture supernatant
ImmunogenSynthetic peptide (N-terminal amino acids Nos. 2-23) of human cytokeratin 2e (MW 65,852).
SpecificityAntigen Recognized Human in Species (tested so far); Bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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