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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH IF

This clone represents an excellent marker to study terminal epidermal differentiation.
Catalog #	C9097-33A
Applications	Suitable for use in Immunoblot and Immunohistochemistry (frozen sections, pretreatment with Triton-X100 is recommended, e.g. 0.2% in PBS for 5 min.; On paraffin sections the microwave method is recommended).
Recommended Dilution	Western Blot: 1:200 (alkaline phosphatase)
	Western Blot: 1:60,000 (ECL)
	Immunofluorescence: 1:200
	Immunohistochemistry: 1:100 (PAP/ABC)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F231
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Hybridoma culture supernatant
Immunogen	Synthetic peptide (N-terminal amino acids Nos. 2-23) of human cytokeratin 2e (MW 65,852).
Specificity	Antigen Recognized Human in Species (tested so far); Bovine
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.