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Anti -Cytokeratin 3, K2P (Keratin K3/K76)
Anti -Cytokeratin 3, K2P (Keratin K3/K76)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
B IH |
|
| Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The cytokeratins are expressed in combinations which characterize the type of epithelium. | | | Catalog # | C9097-34M | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilutions | Western Blot: 1:500 | | Immunohistochemistry (Frozen): 1:50 | | Immunohistochemistry (Paraffin): 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2Q1040 | | Host | Mouse | | Source | Rabbit | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. | | Purity | Purified from by ion exchange chromatography. | | Immunogen | Rabbit corneal epithelial keratin | | Specificity | Recognizes the 64kD polypeptide (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. | | Species Crossreactivity | Human, bovine and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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