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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 3, K2P (Keratin K3/K76)

Anti -Cytokeratin 3, K2P (Keratin K3/K76)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IH
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The cytokeratins are expressed in combinations which characterize the type of epithelium.
Catalog #C9097-34M
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:500
Immunohistochemistry (Frozen): 1:50
Immunohistochemistry (Paraffin): 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1040
HostMouse
SourceRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by ion exchange chromatography.
ImmunogenRabbit corneal epithelial keratin
SpecificityRecognizes the 64kD polypeptide (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
Species CrossreactivityHuman, bovine and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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