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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH FC

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9 – 7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium.
Catalog #	C9097-35A
Applications	Suitable for use in Immunocytochemistry, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Frozen and tissues. Avidin-biotinylated horseradish peroxidase complex (ABC) as a detection reagent.
	Flow Cytometry: 1:25-1:200
	Western Blot: 1:100-1:1000
	Optimal dilutions to be determined by the researcher.
Hybridoma	Hybridization of SP2/0 mouse myeloma cells with spleen cells from Balb/c mice immunized with a cytokeratin preparation extracted from human esophagus.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4A37
Host	Mouse
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Cytokeratin 4.
Specificity	Specifically recognizes cytokeratin 4 in non-cornified squamous epithelia, including cornea and transitional epithelium.
Species Crossreactivity	Human, feline, canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.