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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH FC

Catalog #	C9097-35B
Cytokeratin 4 is mostly present in combination with cytokeratin 13. In contrast to cytokeratin 13, the superficial cells of the cornea are reactive with cytokeratin 4. Also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells. Polypeptide reacting	59kD cytokeratin 4.
Applications	Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Frozen tissue, cell suspensions): 1:5-1:10
	Optimal dilutions to be determined by the researcher.
Positive Control	Squamous cell carcinomas
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F132
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 0.1% sodium azide. Dilute further in PBS, 1% BSA, 0.1% sodium azide.
Purity	Supernatant
Immunogen	Human esophagus
Specificity	Recognizes human cytokeratin 4 in non-cornifying squamous epithelia. Species Crossreactivity: mammal, mouse, rat, rabbit, feline and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.