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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 4 (Keratin, Type II Cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, Type-II Keratin Kb4, KRT4, CYK4)

Anti -Cytokeratin 4 (Keratin, Type II Cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4,
Type-II Keratin Kb4, KRT4, CYK4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Catalog #C9097-35C
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunohistochemistry (paraffin, frozen): 2-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K181
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenCytokeratin from human esophagus.
SpecificityRecognizes human Cytokeratin 4. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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