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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 4 (Cytokeratin-4, CK 4, CK4, CK-4, CYK4, FLJ31692, Keratin 4, Keratin-4, Keratin Type II Cytoskeletal 4, K4, KRT4)

Anti -Cytokeratin 4 (Cytokeratin-4, CK 4, CK4, CK-4, CYK4, FLJ31692, Keratin 4, Keratin-4,
Keratin Type II Cytoskeletal 4, K4, KRT4)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
Intermediate filaments are protein polymers which, together with actin filaments and microtubules, form the cytoskeleton of cells. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Expression of the genes encoding these stratification-related cytokeratins starts already in the basal cell layer and does not depend on vertical differentiation and detachment from the basal lamina. Results also show that simple epithelial and stratification-related cytokeratins can be coexpressed in basal cell layers of certain stratified epithelia such as esophagus (1). Mucous keratin CK4 is known to appear in epidermis under certain abnormal conditions. a dose-response study showed that the de novo appearance of K4 can be utilized as a sensitive test for retinoid bioactivity in epidermis in vivo (2).
Catalog #C9097-35F
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human CK-4.
SpecificityRecogizes human Cytokeratin 4. Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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