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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 5 (Cytokeratin-5, CK 5, CK5, CK-5, 58kD Cytokeratin, DDD, EBS 2, EBS2, Keratin 5 (Epidermolysis Bullosa Simplex Dowling Meara/Kobner/Weber Cockayne Types), Keratin-5, Keratin Type II Cytoskeletal 5, K5, KRT5, KRT5A)

Anti -Cytokeratin 5 (Cytokeratin-5, CK 5, CK5, CK-5, 58kD Cytokeratin, DDD, EBS 2, EBS2,
Keratin 5 (Epidermolysis Bullosa Simplex Dowling Meara/Kobner/Weber Cockayne Types),
Keratin-5, Keratin Type II Cytoskeletal 5, K5, KRT5, KRT5A)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Supernatant B IH IC
Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. The mitotically active basal layers of most stratified squamous epithelia express 10 to 30% of their total protein as keratin. The two keratins specifically expressed in these cells are the type II keratin CK-5 and its corresponding partner, type I keratin CK-14, both of which are essential for the formation of 8-nm filaments (1). CK-5 and calretinin have been useful in different studies as immunohistochemical markers suggestive of mesothelioma, and their expression is analyzed for the histological differential diagnosis with adenocarcinomas, especially when confronting with metastatic tumors of unknown origin (2). Loss-of-function mutations in the keratin 5 gene (KRT5) affected family members and in six unrelated patients with Dowling-Degos disease (DDD), an autosomal dominant genodermatosis. This suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).
Catalog #C9097-37A2
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50-1:100
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human CK-5.
SpecificityRecogizes human Cytokeratin 5 at ~59kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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