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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 5,6

Anti -Cytokeratin 5,6


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Clone Host Grade Applications
Monoclonal Mouse Purified IH
Cytokeratin 5 and 6 are known as high molecular weight cytokeratins (58kD and 56kD respectively) and are part of the basic (type II) subfamily of cytokeratins. These two cytokeratins are closely related and they are found in non-keratinizing strati ed squamous epithelia, epithelia of trachea, apocrine and sweat glands of skin, epidermis, hair follicles and mammary glands. (2)
Catalog #C9097-37F
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:50-1:200. Formalin- xed, paraf n-embedded tumor tissue studies, and may be used to distinguish squamous carcinoma from adenoma. Requires high temperature antigen retrieval using 0.01M EDTA solution; boil for 15 minutes, then cool down for at least 15-20 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
Positive Control TissueSkin
Expected Staining PatternCytoplasmic
Epitope RetrievalRequired EDTA, pH8.0
Enzyme DigestionNot required
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3G123
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
ImmunogenPuri ed Cytokeratin 5 and 6.
SpecificityStains basal cells and part of the stratum spinosum in normal squamous epithelium and part of the basal layer of prostate gland epithelium. It does not stain other normal simple layer glandular epithelium. (1)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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