| Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9–7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. |
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| Catalog # | C9097-37H |
| Applications | Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:100-1:1000 |
| Flow Cytometry: 1:100-1:200 |
| Immunohistochemistry (Frozen and Paraffin-embedded sections): 1:100-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Hybridization of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with cytokeratins from a human lung cancer cel line (MR21). |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 4A38 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Cytokeratins from human lung cancer cell line (MR21) |
| Specificity | A broadly reacting cytokeratin antibody recognizing virtually all epithelial tissues and carcinomas. Species Crossreactivity: Human, mouse, rat, hamster, rabbit, canine, porcine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
