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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH IF

Catalog #	C9097-37H1
Applications	Suitable for use in Immunofluorescence, Immunoblot and Immunohistochemistry (frozen sections; paraffin sections, Proteolytic treatment with pronase or trypsin required; Formalin fixation may cause antigen denaturation or masking. Therefore, protease digestion is necessary prior to antibody application).
Recommended Dilution	Immunohistochemistry: 1:5-1:10
Positive Control	Skin, cervix.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F295
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Culture supernatant
Immunogen	Lung cancer cell preparation.
Specificity	Directed against the 58kD and 52.2kD cytokeratin proteins (cytokeratins 5 and 8), reacts with all epithelial cell types. No reaction is observed in non-epithelial tissues. Can be used for the discrimination of carcinomas from non-epithelial tumors such as lymphomas, sarcomas and neural tumors. Species Crossreactivity: Human, most mammals including mouse, rat, hamster, rabbit, canine and porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.