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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 5,8 (Biotin)

Anti -Cytokeratin 5,8 (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH FC
Catalog #C9097-37K1
ApplicationsSuitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material; Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application).
Recommended DilutionImmunohistochemistry: 1:10-1:20; Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8°C).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F173
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid. Conjugated to Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cytokeratin 8, purified from SDS PAGE.
SpecificityReactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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