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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 6

Anti -Cytokeratin 6


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Reactivities on Cultured A-431, Detroit 562, SCC-12 Cell Lines (tested so far).
Catalog #C9097-38D
ApplicationsSuitable foruse in immunohistochemistry (frozen tissue and paraffinembedded material (P) after formalin fixation; microwave treatment improves performance) and Immunoblot (Western Blot).
Recommended DilutionImmunohistochemistry: 1:10 with PBS; Incubation Time 1 hr at RT, extended with (P).
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5F222
FormSupplied as a lyophilized powder. Reconstitute to a final volume of 1ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenCytokeratin 6 of human callus cytoskeletal preparation.
SpecificityAntigen Recognized in Human, mouse species (tested so far). Excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions). Polypeptide Reacting Mr 56,000 polypeptide (cytokeratin 6) of human squamous epithelia.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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