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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Reactivities on Cultured A-431, Detroit 562, SCC-12 Cell Lines (tested so far).
Catalog #	C9097-38D
Applications	Suitable foruse in immunohistochemistry (frozen tissue and paraffinembedded material (P) after formalin fixation; microwave treatment improves performance) and Immunoblot (Western Blot).
Recommended Dilution	Immunohistochemistry: 1:10 with PBS; Incubation Time 1 hr at RT, extended with (P).
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F222
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder. Reconstitute to a final volume of 1ml.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytokeratin 6 of human callus cytoskeletal preparation.
Specificity	Antigen Recognized in Human, mouse species (tested so far). Excellent marker for non-keratinized squamous epithelia and proliferating cells of epidermis (e.g. within psoriatic lesions). Polypeptide Reacting Mr 56,000 polypeptide (cytokeratin 6) of human squamous epithelia.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.