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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IH

Cytokeratin 7 was found to occur in the columnar and glandular epithelium of the lung, cervix, breast, bile ducts, larger collecting ducts of the kidney and in mesothelium, but is absent from gastrointestinal epithelium, hepatocytes and myoepithelium. Several types of adenocarcinomas of the gastrointestinal tract and of the urinary tract are negative, while adenocarcinomas of the mammary gland, ovary and lung are positive.
Catalog #	C9097-39A1
Applications	Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and paraffin-embedded sections; Protease Digestion: 0.5% Pepsin)
Recommended Dilution	Immunohistochemistry: 1:5-1:10
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F282
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Culture supernatant
Immunogen	Isolated From Cell line T24 (human bladder carcinoma).
Specificity	Antigen Recognized Human, hamster, mouse in Species (tested so far). Antigen Isolated From Cell line T24 (human bladder carcinoma). Recommended for Glandular epithelium of the breast. Decorates specific subtypes of adenocarcinoma. In immunoblot, reactive with the 54kD cytokeratin 7 protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.