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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 7

Anti -Cytokeratin 7

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Cytokeratin 7 was found to occur in the columnar and glandular epithelium of the lung, cervix, breast, bile ducts, larger collecting ducts of the kidney and in mesothelium, but is absent from gastrointestinal epithelium, hepatocytes and myoepithelium. Several types of adenocarcinomas of the gastrointestinal tract and of the urinary tract are negative, while adenocarcinomas of the mammary gland, ovary and lung are positive.
Catalog #C9097-39B1
ApplicationsSuitable for use in Immunoblot and Immunohistochemistry (frozen tissue and cell suspensions).
Recommended DilutionImmunohistochemistry: 1:5-1:10
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F296
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PurityCulture supernatant
ImmunogenIsolated From Cell line T24 (human tumor cell line)
SpecificitySpecies Crossreactivity: Human, hamster, mouse, rat, canine, porcine. Recommended for Glandular epithelium of the breast. Decorates specific subtypes of adenocarcinoma. In immunoblot, reactive with the 54kD cytokeratin 7 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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