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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 7

Anti -Cytokeratin 7

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas colorectal, hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: Adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with colorectal adeno carcinoma and most cases of hepatocellular carcinoma. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.
Catalog #C9097-39D
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and with Cytological Material. Other applications not tetsed.
Recommended DilutionsImmunohistochemistry (Frozen and Paraffin): 1:50 Dilute with PBS, pH 7.4; Incubation Time 1h at RT, extended with paraffin sections (overnight at 4°C). Protease pretreatment is recommended with paraffin sections prior to antibody application.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F234
HostMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml 40-50% sterile glycerol, ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenCytoskeletal proteins from cultured HeLa cells.
SpecificityRecognizes cytokeratin 7 of human glandular epithelia at ~54kD.
Species CrossreactivityBovine, porcine and sheep. Does not react with canine, rabbit, rat or mouse. Reactivities on Cultured Cell Lines: HeLa, RT112, T24, BT-20, CAMA-1, Detroit 562. Does not react with MCF-7 or HT-29.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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