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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 7

Anti -Cytokeratin 7

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH IC FC
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40kD and 68kD and isoelectric pH between 4.9 7.8, The individual cytokeratin polypeptides are designated 120. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Catalog #C9097-40E
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:100-1:1000
Immunohistochemistry (frozen): 1:100-1:200
Immunocytochemistry: 1:100-1:200
Flow Cytometry: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q1114
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in buffer, 0.09% sodium azide.
PurityPurified
ImmunogenCytokeratins from the human bladder carcinoma cell line T24.
SpecificityRecognizes human Cytokeratin 7 (58kD). Reacts with a subgroup of gladular epithelial and their tumors, as well as transitional epithelium and transitional carcinoma. Species Crossreactivity: mouse, rat, hamster, canine, feline and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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