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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH FC

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Catalog #	C9097-40F
Applications	Suitable for use in Immunoblot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunoblot: 1:100-1:1000
	Immunohistochemistry: 1:100-1:200. Frozen tissue sections fixed with methanol; Paraffin embedded tissue sections pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q1133
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in buffer containing 0.09% sodium azide.
Purity	Purified culture supernatant
Immunogen	Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line
Specificity	Recognizes human cytokeratin 7. Reacts with specific glandular-type epithelia and most carcinomas derived from them.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.