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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 8

Anti -Cytokeratin 8

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH
This antibody is useful to determine if a neoplasm originated from a certain type of epithelial source. Cytokeratin 8 exists on several types of normal and neoplastic epithelia , including many ductal and glanular epithelia such as colon, gastric, small intestine, tracheal, esophageal and transitional epithelium. This antibody may be useful for distinction of metastatic malignant tumors of these epithelial origin.
Catalog #C9097-42D
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Formalin- xed paraf n-embedded or frozen tissue sections. Pepsin digestion or heat induced antigen recovery procedure is required for speci c staining of formalin- xed paraf n-embedded tissue sections. To stain, incubate 3060 minutes at RT or overnight at 4°C. Optimal dilutions to be determined by the researcher.
Recommended Positive ControlColon/ gastric mucosa
Expected Staining PatternCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G125
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityPuri ed
ImmunogenHuman Cytokeratin 8 form HeLa cells.
SpecificityRecognizes human cytokeratin 8 (52.5kD). No crossreactivity to other lament types, including vimentin, desmin, glial brillary acidic protein and neuro lament protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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