Anti -Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Cardiac Autoantigen 2 120kD,
Card2, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, KO)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH IC FC |
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| Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. | | | Catalog # | C9097-42F | | Applications | Suitable for use in Immunohistochemistry, Flow Cytometry, Immunocytochemistry, and Western Blot. Other applications not tested. | | Optimum dilutions to be determined by researcher. | | Recommended Dilution | Immunohistochemistry (frozen sections): 1:50-1:100 with avidin-biotinylated HRP complex (ABC) as detection reagent. | | Western Blot: 1:50-1:500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 4A42 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Cytokeratin 8 | | Specificity | Recognizes human Cytokeratin 8 in glandular epithelial cells of the digestive, respiratory and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. Species crossreactivity: Human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Cardiac Autoantigen 2 120kD, Card2, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, KO) |
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