Anti -Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Cardiac Autoantigen 2 120kD,
Card2, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, KO)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH IC |
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| Keratins, the intermediate filament proteins of epithelial cells, connect to desmosomes, the cell-cell adhesion structures at the surface membrane. Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells (1). Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine (2). While K8/K18 are essential for maintaining structural integrity, there is accumulating evidence indicating that they also exert non-mechanical functions. It has been reported that K8/K18-free hepatocytes from K8-null mice are more sensitive to Fas-mediated apoptosis, in line with an increased Fas density at the cell surface and an altered c-Flip regulation of the anti-apoptotic ERK1/2 signaling pathway (3). | | | Catalog # | C9097-42K | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500 | | Immunoprecipitation: 1:60 | | Immunohistochemistry: 1:100-1:250 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.18M sodium chloride, 0.25% sodium azide, 0.25mM EDTA, 25mM Citric Acid, 0.5% BSA, 40% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic pathway corresponding to residues on the N-terminus of human CK-8. | | Specificity | Recognizes human Cytokeratin 8. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Cardiac Autoantigen 2 120kD, Card2, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, KO) |
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