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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 9

Anti -Cytokeratin 9

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts.
Catalog #C9097-43A
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry (frozen tissue for paraffin-embedded sections, microwave treatment and in some cases protease digestion is recommended). The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
Optimal dilutions to be determined by the researcher.
Recommended DilutionWestern Blot: 1:25-1:300 (chemiluminescence, AP/ECL).
Immunofluorescence Microscopy (Frozen): 1:10
Immunohistochemistry: 1:20 (PAP/ABC).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1+IgG3
Clone No5F236, 5F237
HostMouse
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityHybridoma culture supernatant
ImmunogenSynthetic peptides [(1) aa450-477 and (2) N-terminal aa4-28] of human cytokeratin 9 (MW 62,129).
SpecificityRecognizes human Cytokeratin 9 (in mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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