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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 9

Anti -Cytokeratin 9


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts.
Catalog #C9097-43A
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry (frozen tissue for paraffin-embedded sections, microwave treatment and in some cases protease digestion is recommended). The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
Optimal dilutions to be determined by the researcher.
Recommended DilutionWestern Blot: 1:25-1:300 (chemiluminescence, AP/ECL).
Immunofluorescence Microscopy (Frozen): 1:10
Immunohistochemistry: 1:20 (PAP/ABC).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No5F236, 5F237
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PurityHybridoma culture supernatant
ImmunogenSynthetic peptides [(1) aa450-477 and (2) N-terminal aa4-28] of human cytokeratin 9 (MW 62,129).
SpecificityRecognizes human Cytokeratin 9 (in mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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