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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 8,18

Anti -Cytokeratin 8,18

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH
Catalog #C9097-44A
ApplicationsSuitable for use in Immunoblot and Immunohistochemistry (frozen and paraffin-embedded tissue, Proteolytic treatment with pepsin or pronase).
Recommended DilutionImmunohistochemistry: 1:5-1:10, dilute immediately before use.
Positive ControlGlandular epithelium
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F272
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PurityAscites
ImmunogenIsolated from human breast cancer carcinoma cell line MCF-7.
SpecificitySpecies Crossreactivity: Human, No significant reaction with mouse or rat. Recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. This clone is directed against the 52.2kD cytokeratin 8 (originally reported also to 45kD cytokeratin 18) in immunoblot. Epitope has been mapped to aa353-367 on alpha helical rod domain of cytokeratin 8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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