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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 8,18

Anti -Cytokeratin 8,18

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum E B IH IC
Cytokeratins are a family of water-insoluble intermediate laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1-20) in both normal and malignant epithelial cell lines. Speci c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. Cytokeratins may also be clinically signi cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors.
Catalog #C9097-44G
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:2000
Immunohistochemistry (Frozen): 1:100. Incubate for 1hr at RT.
Immunohistochemistry (Paraffin): 1:50. Incubate for >1hr at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceBovine
ConcentrationNot Determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySerum
ImmunogenCytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18.
SpecificityRecognizes bovine Cytokeratin 8,18 of simple type epithelium at ~52.5kD and 45.5kD respectively. Detected all adeno-Ca tested, undifferentiated CA, hepatocellular CA and cervix Ca tumors. Species Crossreactivity: human, rat, mouse, Syrian hamster, Xenopus laevis, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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