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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum E B IH IC

Cytokeratins are a family of water-insoluble intermediate laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1-20) in both normal and malignant epithelial cell lines. Speci c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. Cytokeratins may also be clinically signi cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors.
Catalog #	C9097-44G
Applications	Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Western Blot: 1:2000
	Immunohistochemistry (Frozen): 1:100. Incubate for 1hr at RT.
	Immunohistochemistry (Paraffin): 1:50. Incubate for >1hr at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Bovine
Concentration	Not Determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Serum
Immunogen	Cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18.
Specificity	Recognizes bovine Cytokeratin 8,18 of simple type epithelium at ~52.5kD and 45.5kD respectively. Detected all adeno-Ca tested, undifferentiated CA, hepatocellular CA and cervix Ca tumors. Species Crossreactivity: human, rat, mouse, Syrian hamster, Xenopus laevis, chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.