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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH FC

Cytokeratin Type II constitutes the Type II intermediate filaments (IFs) of the intracytoplasmatic cytoskeleton, which is present in all mammalian epithelial cells. The type 2 cytokeratins consist of basic or neutral, high molecular weight proteins which are arranged in pairs of heterotypic Type I and Type II keratin chains in vivo, coexpressed during differentiation of simple and stratified epithelial tissues.
Catalog #	C9097-61E
Applications	Suitable for use in Flow Cytometry, ELISA, Western Blot, Cell sorting and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (frozen tissue and cytological material): 10-20ug/ml; dilute with PBS, pH 7.4; Incubation Time: 1 hr at RT.
	Optimal dilutions to be determined by the researcher.
Biotin Coupling Ratio	5mol Biotin/1mol IgG.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F224
Host	Mouse
Source	Human
Concentration	Not determined.
Form	Supplied as a liquid containing 0.09% sodium azide. labeled with Biotin.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytoskeletal proteins from cultured human MCF-7 cells.
Specificity	Recognizes human Cytokeratin Type II. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.