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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Catalog #	C9097-61F
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry (frozen tissue and cytological material).
Recommended Dilution	Immunofluorescence: 1:20-1:40 with PBS, pH 7.4; Incubation Time 1 hr at RT.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F224
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.4. Conjugated to FITC.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Cytoskeletal proteins from cultured human MCF-7 cells.
Specificity	Antigen Recognized in Human, bovine, amphibia. Reactivities on Cultured MCF-7, RT 112, HT-29. Cell lines (tested so far) Detroit 562, RPMI 2650, SSC-12 bovine BMGE+H, BMGE-H, MDBK. Tumors specifically detected
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.