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You are here:Home » Kits and Assays » Kits for ELISA (Infectious Disease), BioAssay™ » Cytomegalovirus, Human, IgG, BioAssay™ ELISA Kit (CMV)

Cytomegalovirus, Human, IgG, BioAssay™ ELISA Kit (CMV)

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Specifications

The United States Biological Cytomegalovirus, Human, IgG, BioAssay™ ELISA Kit is intended for use in evaluating serologic status to cytomegalovirus (CMV) infection.
Catalog #C9098-03
Cytomegalovirus is a herpes virus and a leading biological factor causing congenital abnormalities and complications among those who receive massive blood transfusions and immunosuppressive therapy. About half of pregnant women who contract a primary infection spread the disease to their fetus. When acquired in-utero, the infection may cause mental retardation, blindness, and/or deafness.
Serological tests for detecting the presence of antibody to CMV can provide valuable information regarding the history of previous infection, diagnosis of active or recent infection, as well as in screening blood for transfusions in newborns and immuno-compromised recipients. United States Biological ELISA CMV IgG is an accurate serologic method to detect CMV antibody for identification of CMV infection.
Purified CMV antigen is coated on the surface of microwells. Diluted serum is added to wells, and the CMV IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
Sample size 5ul
Specificity 98%
Sensitivity 97%
Total Time ~75 minutes
Kit Components:
C9098-03B: Microtiter Plate, 1x96 wells
C9098-03C: Enzyme Conjugate, 1x12ml
C9098-03D: Negative Calibrator (0IU/ml), 1x150ul
C9098-0E: Cut-off Calibrator (1.2IU/ml) 1x150ul
C9098-03F: Positive Calibrator I (6IU/ml), 1x150ul
C9098-03H: Positive Calibrator II (18IU/ml), 1x150ul
C9098-03J: Negative Control (<1IU/ml), 1x150ul
C9098-03K: Positive Control (1.2-18IU/ml), 1x150ul
C9097-03L: Wash Buffer,10x, 1x100ml
C9097-03M: Sample Diluent, 1x22ml
C9097-03N: TMB, 1x12ml
C9097-03P: Stop Solution (2N HCl), 1x12ml
Storage and Stability:
Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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