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You are here:Home » Antibodies » Abs to Infectious Disease CMV » Anti -Cytomegalovirus, Glycoprotein B (CMV) (HRP)

Anti -Cytomegalovirus, Glycoprotein B (CMV) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Highly Purified E B IC IF
Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human Herpes virus 5 (HHV-5). Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906aa in length, with a MW of ~100kD.
Catalog #C9100-21F
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:200-1:1000
Immunohistochemistry: 1:20-1:200
Western Blot: 1:20-1:200
Indirect Immunofluorescence: >1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration1-2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. No stabilizing proteins have been added. Labeled with HRP.
PurityPurified IgG fraction to a purity of >95%.
ImmunogenPurified virions of strain AD169.
SpecificityRecognizes Cytomegalovirus, Glycoprotein B. Crossreactivity: Does not react with HSV, VZV, HEp-2 cells and human fibroblasts by indirect immunofluorescence or IFA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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