ELISA Kit for Antibody to Cytomegalovirus (CMV) is an in vitro enzyme immunoassay for the detection of Anti-CMV antibodies IgM in human serum or plasma. It is for diagnosis of early infection and epidemic survey.
Principle
This kit uses capture ELISA method to detect anti-CMV antibodies IgM in serum or plasma. The anti-human IgM (mu chain) antibody is coated on the multi-wells plate. The HRP conjugated recombinant CMV antigen serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-CMV antibodies IgM in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of CMV infection.
Kit Components
1. Antibody Coated Microwell Plate, 1x96 wells 2. Enzyme Conjugant 3. Negative Control Serum 4. Positive Control Serum 5. Concentrated Wash Buffer (20x) 6. Substrate A 7. Substrate B 8. Stop Solution
Storage and Stabilty
Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.