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You are here:Home » Antibodies » Antibodies-Nucleotides » Anti -Cytosine, 5-Methyl

Anti -Cytosine, 5-Methyl


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IP IH IC IF
Catalog #C9111
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and immunofluorescence. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:500
Immunoprecipitation: 25-100ug/ml
Immunohistochemistry (Frozen and paraffin sections): 10ug/ml. Fixation in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining of both nuclear, cytoplasmic and membrane components as well as preservation of morphological detail.
Immunocytochemistry: 10ug/ml
Immunofluorescence: 10ug/ml
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 40% glycerol. No preservative added.
PurityPurified by Protein G affinity chromatography.
Immunogen5-Methyl Cytosine (KLH).
SpecificityRecognizes 5-Methyl Cytosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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