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You are here:Home » Antibodies » Abs to Cells, Blood Components » Anti -Cytotoxic Cells, Nonspecific

Anti -Cytotoxic Cells, Nonspecific

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP FC
Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32kD membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
Catalog #C9127
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Inhibition Assay. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation. Other applications not tested.
Recommended DilutionWestern Blot: 10ug/ml; Detects a 32kD protein representing nonspecific cytotoxic cell receptor protein.
Inhibition Assay: Has been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No4i269
HostMouse
Concentration~1mg/ml
FormSupplied in a liquid buffer with 1mg/ml BSA and 0.05% sodium azide.
PurityPurified IgM
ImmunogenPurified NCC cells from catfish.
SpecificityRecognizes NCC. Crossreacts with Bovine, Canine, Equine, Porcine, Rat, Tilapia and Catfish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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