| Catalog # | CS005-005 |
| Monoclonal Antibody Production Total Time | ~4 months. |
| * Free peptide designing * Immunization of 5 Balb/c mice * Fusion of spleen cells with SP2/0 myeloma cells. * ELISA screening for up to 3,000 clones. * Free Western blot screening for protein antigen * Guaranteed IgG isotype * Progress Tracking * ELISA positive guaranteed or your money back. Phase I *Immunization and boosts of 5 Balb/c mice with standard immunization protocol. *ELISA evaluation of titer prior to selection for fusion. *Approximately 3-4mg protein immunogen or 2mg conjugated peptide immunogen and 0.5mg free peptide are required. |
| Phase II *Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells. *Supernatants from the growing hybridoma wells will be screened by ELISA. 10 clones will be sent to customer's lab for evaluation. |
| Phase III *2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped and expanded. |
| Additional Services | Peptide synthesis and conjugation |
| Peptide synthesis without conjugation |
| Peptide conjugation |
| Additional clones (>10) in Phase II |
| Negative screening |
| Extra screening with IHC |
| Additional clones (>2) in Phase III |
| Cell-line adaptation to serum free medium |
| Cell banking and store in 10 ampules after first two months |
| Ascites production |
| Antibody purification by Protein G or ammonium persulfate for IgM (10 mice minimum) |
| Antibody culture in bioreactor |
| Antibody culture in flask |
| Antibody purification by protein G from one liter of medium |
| Antibody purification by protein G from ascites (20mg) |
| Antibody epitope mapping by peptide library |
| Antibody conjugation with FITC |
| Antibody conjugation with HRP |
| Antibody biotinylation |
| Client retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
