Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Custom Services » Custom Antibodies, Monoclonal » Custom Monoclonal Antibodies (Standard Protocol, 120 Days)

Custom Monoclonal Antibodies (Standard Protocol, 120 Days)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Catalog #CS005-005
Monoclonal Antibody Production Total Time ~4 months.
* Free peptide designing * Immunization of 5 Balb/c mice * Fusion of spleen cells with SP2/0 myeloma cells. * ELISA screening for up to 3,000 clones. * Free Western blot screening for protein antigen * Guaranteed IgG isotype * Progress Tracking * ELISA positive guaranteed or your money back. Phase I *Immunization and boosts of 5 Balb/c mice with standard immunization protocol. *ELISA evaluation of titer prior to selection for fusion. *Approximately 3-4mg protein immunogen or 2mg conjugated peptide immunogen and 0.5mg free peptide are required.
Phase II *Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells. *Supernatants from the growing hybridoma wells will be screened by ELISA. 10 clones will be sent to customer's lab for evaluation.
Phase III *2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped and expanded.
Additional ServicesPeptide synthesis and conjugation
Peptide synthesis without conjugation
Peptide conjugation
Additional clones (>10) in Phase II
Negative screening
Extra screening with IHC
Additional clones (>2) in Phase III
Cell-line adaptation to serum free medium
Cell banking and store in 10 ampules after first two months
Ascites production
Antibody purification by Protein G or ammonium persulfate for IgM (10 mice minimum)
Antibody culture in bioreactor
Antibody culture in flask
Antibody purification by protein G from one liter of medium
Antibody purification by protein G from ascites (20mg)
Antibody epitope mapping by peptide library
Antibody conjugation with FITC, HRP, or Biotin
Client retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality.
Client retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links