| USDA-Registered Polyclonal Production |
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| United States Biological is known for more than the thousands of antibodies offered in our catalog. Our antibodies are produced in state of the art facilities in the US. |
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| Catalog # | CS010-010 |
| We maintain current USDA certification as a qualified animal research facility, and comply with the Animal Welfare Act. We follow guidelines set forth by the National Institute of Health (NIH) and have established an Institutional Animal Care and Use Committee (IACUC) to review all production protocols prior, during and after all client programs. |
| Antibody Program | A typical standard protocol includes animal purchase, housing and care, all immunizations, bleeds and exsanguinations as per our standard protocol formats. Your project may require different specifications and, therefore, prices will vary. Our costs are quite reasonable. Although we will conduct single animal programs, the additional costs to add a second animal can produce a significantly greater yield and likelihood for success. |
| Standard 2 Rabbit Protocol | Standard protocol pricing includes animal purchase, housing and care up to 49 days, all immunizations, bleeds and exsanguinations as per the following schedule: |
| Pre-Peptide Consultation if necessary |
| Required, but not included in price | Peptide Production |
| Peptide Conjugation |
| Day 0 Pre-immunization bleed Antigen Injection (s.c and i.m; CFA; 200ug) Day 14 Antigen Injection (IFA; 100ug) Day 28 Antigen Injection (IFA; 100ug) Day 35 First Bleed (20-25ml/rabbit) ELISA Day 42 Antigen Injection (IFA; 100ug) Day 49 Final Production Bleed (~40-50ml/rabbit) ELISA Return of antisera and peptides |
| Continuation (extra cost) | Day 63 Antigen Injection (IFA; 100ug) |
| Day 77 Third Bleed (20-25ml/rabbit) |
| Antigen Requirements | Per Animal Minimum Recommended |
| Chickens 250ug 1mg |
| Rabbits 500ug 2mg |
| Goats 2.5mg 5mg |
| Sheep 2.5mg 5mg |
| The amount of antigen required varies from a minimum as shown for the standard protocol to a maximum when additional injections might be needed to boost antibody production or to continue a maintenance program longer than the stated time. Affinity columns will also require additional antigen. |
| Antibody Quotation Worksheet | For a quotation, please supply the following information: |
| 1. Antigen name or peptide sequence |
| 2. Type of protein o Natural o Recombinant o Synthetic o Purity ___% o MW. ___ |
| 3. Conjugation o BSA o KLH o Ovalbumin o Thyroglobulin |
| 4. Number of animals required |
| 5. Immunization protocol o Customer Supplied o United States Biological Supplied |
| Important Note | Standard 35 day projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$195/rabbit/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instructions are properly followed. |
| Optional Antibody Services (by quotation) | |
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| Purification | |
| Rabbit polyclonal antibodies can be affinity purified. This is best suited for when serum alone often has too much non-specific interference. |
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| ELISA | |
| Each antibodies (from 2 rabbits) will be tested in a standard ELISA format for titer. |
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| Western Blot | Purified antibody is tested in Western Blot against a variety of normal tissue, tumor tissue, and cell line lysates (see below). Just select which lysates you would like to have run. A full panel of 14 lysates of your choosing is provided, which yields a more comprehensive expression pattern and allows for testing in human, mouse, rat tissue and cell lines. Scans of the blot in an electronic image are supplied. |
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| Immunohistochemistry | |
| Purified antibodies are suitable for use in Immunohistochemistry. We have a comprehensive variety of normal and tumor tissues, and slides. This service includes testing for five tissues. Electronic images are provided. |
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| Lysates may be supplied by group only. Selection may change | |
| 293 whole cell lysate |
| 3T3 whole cell lysate |
| A431 whole cell lysate |
| A549 whole cell lysate |
| Caco-2 whole cell lysate |
| Daudi whole cell lysate |
| HeLa whole cell lysate |
| HepG2 whole cell lysate |
| HL60 whole cell lysate |
| HT-29 whole cell lysate |
| Jurkat whole cell lysate |
| K562 whole cell lysate |
| MCF7 whole cell lysate |
| MDA-MB-361 whole cell lysate |
| MOLT4 whole cell lysate |
| PC-3 whole cell lysate |
| Raji whole cell lysate |
| Ramos whole cell lysate |
| SK-N-SH whole cell lysate |
| SW1353 whole cell lysate |
| SW480 whole cell lysate |
| T24 whole cell lysate |
| THP-1 whole cell lysate |
| U937 whole cell lysate |
| WI-38 whole cell lysate |
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| Human Tissue Lysate |
| Human brain tissue lysate |
| Human heart tissue lysate |
| Human kidney tissue lysate |
| Human liver tissue lysate |
| Human lung tissue lysate |
| Human pancreas tissue lysate |
| Human small intestine tissue lysate |
| Human spleen tissue lysate |
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| Human Tumor Tissue Lysate |
| Human brain tumor lysate |
| Human breast tumor lysate |
| Human colon normal tissue lysate |
| Human colon tumor tissue lysate |
| Human kidney tumor lysate |
| Human liver normal tissue lysate |
| Human liver tumor tissue lysate |
| Human lung tumor lysate |
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| Mouse Tissue Lysate |
| Mouse adipose tissue lysate |
| Mouse adrenal tissue lysate |
| Mouse bladder tissue lysate |
| Mouse brain tissue lysate |
| Mouse cerebellum tissue lysate |
| Mouse cerebrum tissue lysate |
| Mouse colon tissue lysate |
| Mouse heart tissue lysate |
| Mouse kidney tissue lysate |
| Mouse liver tissue lysate |
| Mouse lung tissue lysate |
| Mouse pancreas tissue lysate |
| Mouse skeletal muscle tissue lysate |
| Mouse skin tissue lysate |
| Mouse small intestine tissue lysate |
| Mouse spleen tissue lysate |
| Mouse stomach tissue lysate |
| Mouse testis tissue lysate |
| Mouse thymus tissue lysate |
| Mouse whole eye tissue lysate |
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| Rat Tissue Lysate |
| Rat adrenal tissue lysate |
| Rat bladder tissue lysate |
| Rat brain tissue lysate |
| Rat cerebellum tissue lysate |
| Rat cerebrum tissue lysate |
| Rat colon tissue lysate |
| Rat eye tissue lysate |
| Rat heart tissue lysate |
| Rat kidney tissue lysate |
| Rat liver tissue lysate |
| Rat lung tissue lysate |
| Rat pancreas tissue lysate |
| Rat skeletal muscle tissue lysate |
| Rat skin tissue lysate |
| Rat small intestine tissue lysate |
| Rat spleen tissue lysate |
| Rat stomach tissue lysate |
| Rat testis tissue lysate |
| Rat thymus tissue lysate |
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| IACUC |
| Antibody programs are produced in a facility which maintains current USDA certification as a qualified animal research facility, and complies with the Animal Welfare Act. Guidelines are set forth by the National Institute of Health (NIH). An established Institutional Animal Care and Use Commitee (IACUC) reviews all production protocols prior, during and after all client programs. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
