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You are here:Home » Custom Services » Custom Services-Antibodies, Polyclonal » Custom Polyclonal Antibodies, Chicken

Custom Polyclonal Antibodies, Chicken

Pricing

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  Outside USA? Please contact your distributor for pricing.

Specifications

Catalog #CS010-011
Chicken IgY It's Evolutionary!
Chicken antibodies offer advantages over traditional rabbit and goat polyclonal antibodies due to the evolutionary difference between these immunoglobulins. Chickens give better antibody response against conserved mammalian antigens, they do not react with rheumatoid factors, bacterial or mammalian Fc receptors and can reduce background reactions due to cross-reactivity of anti-IgG antibodies.
We can supply high specificity anti-IgY secondary reagents against the following conjugates Alkaline Phosphatase (AP), Horseradish Peroxidase (HRP), Fluorescein (FITC), allowing suitability for ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Gel Mobility Shift assays.
Perfect for Immunodiagnostics and Kits due to the high yield compared to rabbits. One egg is capable of producing up to 80mg of antibody!
Standard Chicken Protocol Standard protocol pricing includes animal purchase, housing and care, all immunizations and bleeds and exsaguination as per the following schedule
Day X Pre-immunization bleed (eggs can be collected, too) Day 0 1st Immunization, Freund's complete adjuvant, 50-100ug Day 21 2nd Immunization (Booster), incomplete adjuvant, 50ug Day 42 3rd Immunization (Booster), incomplete adjuvant, 50ug Day 49 Test bleed & Start of egg collection.
Eggs should provide about 50 to100 mg of Ig per yolk
Hens will lay approximately 1 egg per day while on the nest. Hens can go off the nest for several days, hence we recommend 2 or more hens for continued production.
We will require a minimum of 500ug of antigen per hen. We recommend at least 1mg per hen.
Additional Services
Egg Handling
Antigen-BSA Conjugation
Total IgY Purification
Affinity Purification
Important NoteStandard 49 day projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$195/chicken/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instruction are properly followed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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