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You are here:Home » Custom Services » Custom Services-Antibodies, Polyclonal » Custom Polyclonal Antibodies, Goat, Project Extension, Monthly

Custom Polyclonal Antibodies, Goat, Project Extension, Monthly

Pricing

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  Outside USA? Please contact your distributor for pricing.

Specifications

Catalog #CS010-012A
USDA-Registered Polyclonal ProductionUnited States Biological is known for more than the thousands of antibodies offered in our catalog. Our antibodies are produced in state of the art facilities in the US.
We maintain current USDA certification as a qualified animal research facility, and comply with the Animal Welfare Act. We follow guidelines set forth by the National Institute of Health (NIH) and have established an Institutional Animal Care and Use Commitee (IACUC) to review all production protocols prior, during and after all client programs.
A typical standard protocol includes animal purchase, housing and care, all immunizations, bleeds and exsanguination as per our standard protocol formats. Your project may require different specifications and, therefore, prices will vary. Our costs are quite reasonable. Although we will conduct single animal programs, the additional costs to add a second animal can produce a significantly greater yield and likelihood for success.
Standard Goat ProtocolThe schedule for goats is the same as for rabbits, but the immunogen dose is higher. One goat receives the dose of two rabbits. Goats take a little longer to develop titer than rabbits, so they are probably not suited for a rapid antibody development program.
We would take some pre-immune serum, and immunize with CFA to start, and immunize again at week 3 and week 6, with the first bleed taken at week 7.
Additional immunizations are done every four weeks, with bleeds taken one week after the immunization. Standard protocol pricing includes animal purchase, housing and care up to 56 days, all immunizations, bleeds and exsanguination as per the following schedule
Pre-Peptide Consultation if necessary Peptide Production Peptide Conjugation
Day 0 Pre-immunization bleed Antigen Injection (s.c and i.m; CFA; 400ug) Day 21 Antigen Injection (IFA; 200ug) Day 42 Antigen Injection (IFA; 200ug) Day 49 First Bleed (20-25ml/goat) ELISA Day 49 Antigen Injection (IFA; 200ug) Day 56 Final Production Bleed (~40-50ml/goat) ELISA Return of antisera and peptides
Continuation (extra cost)Day 63 Antigen Injection (IFA; 200ug)
Day 77 Third Bleed (20-25ml/goat)
Antigen RequirementsPer Animal Minimum Recommended
Chickens 250ug 1mg
Rabbits 500ug 2mg
Goats 2.5mg 5mg
Sheep 2.5mg 5mg
The amount of antigen required varies from a mininum as shown for the standard protocol to a maximum when additional injections might be needed to boost antibody production or to continue a maintenance program longer than the stated time. Affinity columns will also require additional antigen.
Important NoteStandard projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$295/goat/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instruction are properly followed.
Antibody Quotation WorksheetFor a quotation, please supply the following information:
1. Antigen name or peptide sequence
2. Type of protein o Natural o Recombinant o Synthetic o Purity ___% o MW. ___
3. Conjugation o BSA o KLH o Ovalbumin o Thyroglobulin
4. Number of animals required
5. Immunization protocol o Customer Supplied o United States Biological Supplied
Important NoteStandard projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$295/goat/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instruction are properly followed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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