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You are here:Home » Custom Services » Custom Protein Expression » Custom Protein Expression, Baculovirus

Custom Protein Expression, Baculovirus

Pricing

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Specifications

Catalog #CS090-05
Baculovirus ExpressionUnited States Biological offers a baculovirus recombinant protein expression service. Our high-level baculovirus expression system includes fast turn-around time, online progress tracking, and competitive pricing.
Breakdown of Standard Expression
Phase I* Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning of it into a transfer vector with His-tag.
* Sequencing confirmation of insert.
* Transformation of E. coli with baculoviral DNA and gene of interest.
* Culture of E. coli and purification using mini-prep to produce bacmid (recombinant viral DNA).
* Transfection of sf9 insect cells with bacmid.
* Plaque purification and viral titer assay.
* Small-scale culture to test expression level.
Estimated time: 4-6 weeks
Phase II* Virus stock preparation
* Large-scale culture and infection of insect cells for protein expression.
* Purification from 1 liter of infected insect cell using affinity column of Ni-NTA or other column.
Estimated time: 2 weeks
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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