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You are here:Home » Custom Services » Custom Protein Expression » Custom Protein Expression, E. coli

Custom Protein Expression, E. coli

Pricing

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Specifications

Catalog #CS090
E. coli ExpressionUnited States Biological offers a recombinant protein expression service in E. coli. Our high-level recombinant protein expression system includes scale up as requested, tag removal as requested, fast turn-around time, and competitive pricing.
Phase ICloning of your interest gene into bacterial expression vector with His-tag or other selected tag (such as GST or thioredoxin).
*Amplification of gene of interest out of a customer-supplied vector and subcloning of it into an expression vector. *Verification of the authenticity of the subcloned gene by restriction enzyme digestion and sequencing. *Transformation of recombinant construct into high efficiency expression bacterial strain. *Mini-induction to over-express the target protein. *Test for the expression of the recombinant protein by SDS-PAGE or/and western blot. Estimated time 2~4 weeks
Phase II Large-scale Culture and PurificationUp to 1Liter of bacterial culture will be induced with IPTG and harvested for protein purification.
Protein purification using Ni-NAT (for His-tagged proteins) or glutathione (for GST-tagged proteins) beads at either native or denatured conditions.
Solubility not guaranteed Estimated time 2 weeks


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