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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -D4-GDI (GDP Dissociation Inhibitor, Rho GDP-dissociation Inhibitor 2, Rho GDI 2, Ly-GDI, Rho-GDI beta, ARHGDIB, GDIA2, GDID4, RAP1GN1)

Anti -D4-GDI (GDP Dissociation Inhibitor, Rho GDP-dissociation Inhibitor 2, Rho GDI 2,
Ly-GDI, Rho-GDI beta, ARHGDIB, GDIA2, GDID4, RAP1GN1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
Catalog #D0101
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Whole cell lysate from Daudi or Jurkat cell can be used as positive control and a band of approx. size of 28kD is detected.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4H73
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS 0.02% sodium azide, 0.02% gelatin.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to human D4-GDI.
SpecificityRecognizes human D4-GDI
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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