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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Dab1, phosphorylated (Tyr198) (Disabled homolog 1 Drosophila, Scm, Scr, Scrambler, Yot, Yotari)

Anti -Dab1, phosphorylated (Tyr198) (Disabled homolog 1 Drosophila, Scm, Scr, Scrambler,
Yot, Yotari)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Disabled 1 (Dab1) is an 80kD protein that is encoded by the Disabled 1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 tyrosine 198 is a major site for reelin-induced Src family kinase-mediated phosphorylation in embryonic neurons.
Catalog #D0900-14C
Positive Control Human Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
Form0.05% Sodium Azide; 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human Dab1 that contains tyrosine 198.
SpecificityRecognizes Dab1 when phosphorylated at Tyr198. Species Crossreactivity: Crossreacts with Mouse. Expected to crossreact with Chicken (100% identity with immunogen), Rat (100% identity with immunogen), and human (100% identity with immunogen) due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDisabled homolog 1 (Drosophila), Scm, Scr, Scrambler, Yot, Yotari


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