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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Dab1, phosphorylated (Ser491) (Disabled homolog 1 Drosophila, Scm, Scr, Scrambler, Yot, Yotari)

Anti -Dab1, phosphorylated (Ser491) (Disabled homolog 1 Drosophila, Scm, Scr, Scrambler,
Yot, Yotari)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Disabled 1 (Dab1) is an 80kD protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3b (GSK-3b) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.
Catalog #D0900-15
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 When examining phosphorylation of endogenous Dab1 protein it is recommended that Dab1 protein first be immunoprecipitated.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.1% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography
ImmunogenSynthetic phosphopeptide derived from the region of human Dab1 that contains serine 491.
SpecificityRecognizes human Dab1 when phoshorylated at Ser491. Species sequence homology: Mouse, rat and chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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