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You are here:Home » BioSeparation? » BioSep-Assay Detection, Substrates » DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO

DAB Stable Liquid Substrate 50X Concentrate, 0.0694mM Staining System for HRPO

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Specifications

DAB is widely used for both immunoblotting and immunohistochemical staining techniques. It produces an insoluble end product that is brown in color and insoluble in alcohol. It is supplied as 50X concentrate.
Catalog #D1000
DAB is the most common reagent employed for the immunohistochemical detection of horseradish peroxidase (HRP) labeled probes. DAB powder from different sources may show great variability with respect to purity. Histochemical grade material should be pale tan with no purple hue. The purple material is a DAB oxidation product that binds avidly to heme proteins such as myoglobin, hemoglobin and particularly, catalase. Therefore purple by-products should be avoided for histochemical uses.
United States Biological provides a stable DAB concentrate manufactured from carefully selected reagents. When diluted in appropriate buffers with added hydrogen peroxide, the resulting solution provides excellent results with most immunohistochemical and blotting procedures.
Note DAB is a suspected carcinogen.
Color (Light tan to brown)Passes
Stability (4°C and RT)Passes
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months.
Related ProductsTris HCl Buffer is supplied separately (Cat# D1000-05).
CAS Number91-95-2
In the presence of HRP and hydrogen peroxide, DAB is oxidized to an insoluble brown polymer easily recognized by light microscopy. The polymer is insoluble in most organic solvents allowing the use of xylene based mounting medium. Similar color is obtained on blotting matrices.
Features
Exhibits low background levels
Safe to use - no weighing of hazardous powders
Save preparation time - use right out of the bottle
Ultra-low sensitivity
Minimal lot-to-lot variation
Alternate names3,3? Diaminobenzidine


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