| The 32kD dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2, 3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4). |
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| Catalog # | D1075-02B |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:5000-1:20,0000 |
| Immunoprecipitation: 1:50 |
| Immunohistochemistry: 1:250-1:500 |
| Immunocytochemistry: 1:50-1:100 |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Western Blot: Human brain lysate |
| Immunohistochemistry: Human gastric adenocarcinoma (Paraffin-embedded) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 6k145 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues in the C-terminal of human DARPP-32 |
| Specificity | Recognizes human DARPP-32. Species Crossreactivity: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
