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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DARPP32, NT (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Anti -DARPP32, NT (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1
regulatory subunit 1B, PP1R1B)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
The 32kD dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated(1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1(2,3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (Cdk5), DARPP-32 is converted into an inhibitor of PKA(1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers(4).
Catalog #D1075-02C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000-1:50,000
Immunoprecipitation: 1:50
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive ControlWestern Blot: Rat brain lysate
Immunohistochemistry: Human breast adenocarcinoma (Paraffin-embedded)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k146
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues near the N-terminal of human DARPP-32
SpecificityRecognizes human DARPP-32. Species Crossreactivity: Mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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