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Anti -DARPP32, phosphorylated (Thr34) (Dopamine- and cAMP-regulated Neuronal Phosphoprotein, DARPP-32, FLJ20940, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B)
Anti -DARPP32, phosphorylated (Thr34) (Dopamine- and cAMP-regulated Neuronal
Phosphoprotein, DARPP-32, FLJ20940, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit
1B, PPP1R1B)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Purified |
B |
|
| DARPP-32 (dopamine and cyclic AMPregulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/ | | | threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological | | | and behavioral phenotype (3). | | | Catalog # | D1075-03D | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 11C1022 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. | | Purity | Purified | | Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32. | | Specificity | Recognizes endogenous levels of DARPP-32, phosphorylated at (Thr34). Species Crossreactivity: mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B |
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