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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DARPP32, phosphorylated (Thr34) (Dopamine- and cAMP-regulated Neuronal Phosphoprotein, DARPP-32, FLJ20940, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit 1B, PPP1R1B)

Anti -DARPP32, phosphorylated (Thr34) (Dopamine- and cAMP-regulated Neuronal
Phosphoprotein, DARPP-32, FLJ20940, Protein Phosphatase 1 Regulatory (Inhibitor) Subunit
1B, PPP1R1B)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B
DARPP-32 (dopamine and cyclic AMPregulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/
threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological
and behavioral phenotype (3).
Catalog #D1075-03D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1022
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
PurityPurified
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32.
SpecificityRecognizes endogenous levels of DARPP-32, phosphorylated at (Thr34). Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesProtein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B


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