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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DARPP32, phosphorylated (Thr75) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Anti -DARPP32, phosphorylated (Thr75) (Dopamine and cAMP-regulated Phosphoprotein, Protein
phosphatase 1 regulatory subunit 1B, PP1R1B)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
DARPP-32 is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation. Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1). Alternatively, phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) .
Catalog #D1075-04
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000, detects a band of approximately 32kD in rat brain lysates prepared from caudate slices that had been incubated in okadaic acid.
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsI1904-40J: IgG, Fc (HRP) Pab Gt xRb
Sheep anti rabbit IgG:HRP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 0.01% BSA, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr 75.
SpecificityRecognizes rat DARPP-32, also known as protein phosphatase 1 regulatory subunit 1B (PP1R1B), when phosphorylated at threonine 75. Species Crossreactivity: Human, mouse, bovine, canine, chicken and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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