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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1
regulatory subunit 1B, PP1R1B)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH
Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32kD (DARPP-32), also known as PPP1R1B, is a 23kD protein that anomalously migrates at about 32-35kD on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.
Catalog #D1075-05A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Immunohistochemistry (Paraffin): 8-25ug/ml detects DARPP-32 in rat corpus striatum tissue.
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human DARPP-32.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H163
HostRat
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa51-204 of human DARPP-32 expressed in E. coli (Q9UD71). Species Sequence Homology: human
SpecificityRecognizes DARPP-32. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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