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You are here:Home » Antibodies » Abs to Protein Phosphatases » Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1
regulatory subunit 1B, PP1R1B)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH
Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kD (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about 32-35kD on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.
Catalog #D1075-05A
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Immunohistochemistry (paraffin): 8-25ug/ml detects DARPP-32 in rat corpus striatum tissue.
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human DARPP-32.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS NumberN/A
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H163
HostRat
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human DARPP-32 (aa51-204) Accession # Q9UD71
SpecificityRecognizes mouse DARPP-32. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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