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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DARPP32, phosphorylated (Thr75) (Protein Phosphatase 1 Regulatory Subunit 1B, DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, PPP1R1B)

Anti -DARPP32, phosphorylated (Thr75) (Protein Phosphatase 1 Regulatory Subunit 1B,
DARPP-32, Dopamine- and cAMP-regulated Neuronal Phosphoprotein, PPP1R1B)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IF
DARPP-32 is a dopamine (DA) and cAMP-regulated 32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Catalog #D1075-05B
ApplicationsSuitable for use in Immunofluorescence. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
SpecificityRecognizes human DARPP32 when phosphorylated at Thr75. Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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