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Anti -DARPP32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
Anti -DARPP32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein
phosphatase 1 regulatory subunit 1B, PP1R1B)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B |
|
| DARPP-32 is also known as protein phosphatase 1 regulatory subunit 1B (PP1R1B). DARPP-32 is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation. Phosphorylation at threonine 34 results in converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1). Alternatively, phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) . | | | Catalog # | D1075-06 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000. Detects a band of ~32kD in rat caudate lysates. | | Optimal dilution determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | Not Determined | | Form | Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA, 50% glycerol. | | Purity | Serum | | Immunogen | Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr 34. | | Specificity | Recognizes rat DARPP-32 when phosphorylated at threonine 34. Species Sequence Homology: mouse, canine, human, bovine, chicken, and monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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